Journal: Stem Cell Research & Therapy

Article Title: microRNA-375 released from extracellular vesicles of bone marrow mesenchymal stem cells exerts anti-oncogenic effects against cervical cancer

doi: 10.1186/s13287-020-01908-z

Figure Lengend Snippet: Differential expressions of miR-375 and MELK were detected in cervical cancer. a The heat map of the top 50 differentially expressed genes in GSE7803 microarray. b The heat map of the top 50 differentially expressed genes in GSE63514 microarray. In panels a and b , the X -axis indicates the sample number while the Y -axis represents the gene. The tree diagram on the left indicates the gene expression cluster. Each square represents the expression of one gene in one sample. The histogram on the right shows intensity as a color gradation. c Intersection of differentially expressed genes in cervical cancer. Two circles represent the upregulated genes in cervical cancer-related two microarrays. The intersected region represents the intersection results. d Protein-protein intersection network of differentially expressed genes in cervical cancer. The circle reflects the core degree. e The expression of MELK in a sample at different stages of cervical cancer. The X -axis indicates the sample number while the Y -axis represents the gene. The first box indicates the MELK expression in normal cervical samples while the remaining four boxes present the MELK expression in cervical cancer samples at different stages. f Intersection of regulatory BMSC-EV-derived miRNAs and miRNAs in cervical cancer samples. The three circles represent the results obtained from the mirDIP database, TargetScan database, and previous literature, respectively. g The expression of miR-375 was determined using RT-qPCR in HcerEpic, CaSki, C33A, HeLa, and SiHa cell lines, normalized to U6. h The mRNA expression of MELK was determined using RT-qPCR in HcerEpic, CaSki, C33A, HeLa, and SiHa cell lines, normalized to β-actin. The measurement data are presented as mean ± SD. Multiple groups of data are compared by one-way ANOVA and Tukey’s test. * p < 0.05 compared with the HcerEpic cell line

Article Snippet: Human normal cervical epithelial cells (HcerEpic), human cervical cancer cell lines (CaSki, C33A, HeLa and SiHa), and HEK293T cells were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Microarray, Gene Expression, Expressing, Derivative Assay, Quantitative RT-PCR

Journal: Open Medicine

Article Title: Circ_0051428 targeting miR-885-3p/MMP2 axis enhances the malignancy of cervical cancer

doi: 10.1515/med-2023-0858

Figure Lengend Snippet: Circ_0051428 is overexpressed in cervical cancer. (a) The circ_0051428 expressions in normal and CC tissues were estimated via qRT-PCR. P < 0.0001 vs Normal. (b) The circ_0051428 levels in the CC cell lines (CaSki, C33A, HeLa, and SiHa) as well as in the HcerEpic cells were quantified via qRT-PCR. ** P < 0.01 vs HcerEpic. (c) Circ_0051428 levels within the HeLa and CaSki nuclei and cytoplasm. (d) The circ_0051428 and linear RELB expressions in total cellular RNA incubated with RNase R. ** P < 0.01 vs Control.

Article Snippet: HcerEpic (cat. #GPC0088), a normal human cervical epithelial cell line, was obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Quantitative RT-PCR, Incubation, Control

Journal: Open Medicine

Article Title: Circ_0051428 targeting miR-885-3p/MMP2 axis enhances the malignancy of cervical cancer

doi: 10.1515/med-2023-0858

Figure Lengend Snippet: Circ_0051428 targets miR-885-3p. (a) CircInteractome predicted the binding site between circ_0051428 and miR-885-3p. (b) Luciferase activities in HeLa and CaSki that have a combination of pGL3-circ_0051428 WT/MUT and miR-885-3p mimic/NC had been assessed via the dual luciferase experiment. ** P < 0.01 vs miR-NC. (c) Circ_0051428’s interaction with miR-885-3p was verified using the results of the RIP experiment. ** P < 0.01 vs anti-IgG. (d) The miR-885-3p levels in normal and CC tissues were estimated via qRT-PCR. P < 0.0001 vs Normal. (e) The miR-885-3p levels among the CC cell lines (HeLa and CaSki) as well as in HcerEpic cells were gauged by conducting qRT-PCR. ** P < 0.01 vs HcerEpic. (f) In CC tissues, the correlation of circ_0051428 expression with that of miR-885-3p had been ascertained using Pearson’s correlation coefficient.

Article Snippet: HcerEpic (cat. #GPC0088), a normal human cervical epithelial cell line, was obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Binding Assay, Luciferase, Quantitative RT-PCR, Expressing

Journal: Open Medicine

Article Title: Circ_0051428 targeting miR-885-3p/MMP2 axis enhances the malignancy of cervical cancer

doi: 10.1515/med-2023-0858

Figure Lengend Snippet: mir-885-3p targets MMP2. (a) TargetScan predicted the putative sites between miR-885-3p and MMP2. (b) The target relationship of MMP2 with miR-885-3p was substantiated by the outcomes of the dual luciferase experiment. ** P < 0.01 vs miR-NC. (c) The amount of MMP2 in CC and normal tissues was estimated via qRT-PCR. P < 0.0001 vs Normal. (d) The MMP2 expression in HcerEpic and CC (HeLa and CaSki) cells was quantified via qRT-PCR. ** P < 0.01 vs HcerEpic. (e) The association of miR-885-3p expression in CC tissues with that of MMP2 was ascertained using Pearson’s correlation coefficient.

Article Snippet: HcerEpic (cat. #GPC0088), a normal human cervical epithelial cell line, was obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Luciferase, Quantitative RT-PCR, Expressing